The Segars' Lab
The Howard W. and Georgeanna Seegar Jones Division of Reproductive Sciences & Women’s Health Research
Johns Hopkins University School of Medicine, Department of Gynecology and Obstetrics
Laboratory studies in the Division include basic and translational studies on clinical conditions, such as uterine fibroids, endometriosis, and PCOS. Approaches include 2D and 3D cell culture, murine models using Cre-lox targeted deletion of genes, murine and primate models using CRISPR-Cas9 gene editing, as well as the study of reproductive tissues using current molecular biological approaches.
Our laboratory studies involve active collaborations with investigators in the GYN/OB Department, The Johns Hopkins School of Medicine, and Johns Hopkins University. Collaborators include Professor Xiaoqin Wang, Professor Chris Ross, Professor Richard Huganir, and Assistant Professor Reza Kalhor.
Areas of Focus
3D Organoid Culture of Endometriosis using patient derived, immortalized, endometrial and stromal cells to further understand somatic cancer-driver mutations
Translational studies focusing on the development and pathophysiology of uterine fibroids
Understanding genes and regulatory pathways required for granulosa cells to respond to gonadotropins and other hormone signaling
Assisted Reproductive Technology
Using embryological and andrological techniques, like IVF and ICSI, to generate transgenic marmoset models